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ISO/TS 20131-1:2018

Current Revision

Soil quality - Easy laboratory assessments of soil denitrification, a process source of N2O emissions - Part 1: Soil denitrifying enzymes activities

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This document specifies a laboratory test for characterizing the denitrifying enzyme activities in soils[5]. It globally characterizes the transformation of the nitrate form to the nitrous oxide and dinitrogen forms. This method was first proposed by Reference [5] with the acronym DEA for Denitrifying Enzyme Activities. It is a standardized technique used in numerous scientific studies.

DEA estimates the process of denitrification of fresh soil samples incubated under optimal conditions of substrates (nitrate and carbon sources) and environment (anaerobiosis, controlled temperature) for the denitrification process. The de novo enzyme synthesis is blocked by the use of chloramphenicol. DEA is believed to represent the size of the denitrifying enzyme pool present in the soil sample at the time of sample collection.

This test is performed in laboratory on a composite of sieved samples collected at the plot scale. It can be performed on all types of soils sampled all over the year.


SDO ISO: International Organization for Standardization
Document Number ISO/TS 20131
Publication Date Not Available
Language en - English
Page Count
Revision Level
Supercedes
Committee ISO/TC 190/SC 4
Publish Date Document Id Type View
Not Available ISO/TS 20131-1:2018 Revision