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ASTM E388-04

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Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers

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1.1 This test method covers the testing of the spectral bandwidth and wavelength accuracy of fluorescence spectrometers that use a monochromator for emission wavelength selection and photomultiplier tube detection. This test method can be applied to instruments that use multi-element detectors, such as diode arrays, but results must be interpreted carefully. This test method uses atomic lines between 250 nm and 1000 nm.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


This test method covers the testing of the spectral bandwidth and wavelength accuracy of fluorescence spectrometers that use a monochromator for emission wavelength selection and photomultiplier tube detection. The method can be applied to instruments that use multi-element detectors, such as diode arrays, but results must be interpreted carefully. Atomic lines between 250 nm and 1000 nm are used in the method. The difference between the apparent wavelength and the known wavelength for a series of atomic emission lines is used as a test for wavelength accuracy. The apparent width of some of these lines is used as a test for spectral bandwidth.


SDO ASTM: ASTM International
Document Number E388
Publication Date Nov. 1, 2004
Language en - English
Page Count 3
Revision Level 04
Supercedes
Committee E13.01
Publish Date Document Id Type View
Nov. 1, 2004 E0388-04 Revision
Jan. 1, 2023 E0388-04R23 Reaffirmation
May 1, 2015 E0388-04R15 Reaffirmation
Oct. 1, 2009 E0388-04R09 Reaffirmation
Oct. 10, 1998 E0388-72R98 Reaffirmation