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ASTM E2525-08

Historical Revision

Standard Test Method for Evaluation of the Effect of Nanoparticulate Materials on the Formation of Mouse Granulocyte-Macrophage Colonies

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1.1 This test method provides a protocol for quantitative analysis of the effect of nanoparticulate materials in physiologic solution on granulocyte-macrophage colony-forming units.

1.2 This test method employs murine bone marrow hematopoietic stem cells which proliferate and differentiate to form discrete cell clusters or colonies which are counted.

1.3 This test method is part of the in vitro preclinical characterization cascade for nanoparticulate materials for systemic administration in medical applications.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Stem cells of hematopoietic origin are pluripotential and may be particularly sensitive to the effects of stimulation by nanoparticulate materials.

The effect of particles on macrophage responses has an extensive history and can be assessed by Practice F 1903. The test method described here will assess the effect on stem cells which can be progenitor cells to the macrophage line.

SDO ASTM: ASTM International
Document Number E2525
Publication Date Feb. 1, 2008
Language en - English
Page Count 5
Revision Level 08
Supercedes
Committee E56.03
Publish Date Document Id Type View
July 1, 2022 E2525-22 Revision
Sept. 1, 2013 E2525-08R13 Revision
Feb. 1, 2008 E2525-08 Revision