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ASTM E2196-07

Historical Revision

Standard Test Method for Quantification of <emph type="bdit">Pseudomonas aeruginosa</emph > Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor

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1.1 This test method is used for growing a repeatable Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm for viable cells.

1.2 In this test method, biofilm population density is recorded as log colony forming units per surface area.

1.3 Basic microbiology training is required to perform this test method. This standard does not claim to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety practices and determine the applicability of regulatory limitations prior to use.


Bacteria that exist in a biofilm are phenotypically different from suspended cells of the same genotype. The study of biofilm in the laboratory requires protocols that account for this difference. Laboratory biofilms are engineered in growth reactors designed to produce a specific biofilm type. Altering system parameters will correspondingly result in a change in the biofilm. The purpose of this method is to direct a user in the laboratory study of biofilms by clearly defining each system parameter. This method will enable a person to grow, sample, and analyze a laboratory biofilm.

SDO ASTM: ASTM International
Document Number E2196
Publication Date April 1, 2007
Language en - English
Page Count 5
Revision Level 07
Supercedes
Committee E35.15
Publish Date Document Id Type View
April 1, 2023 E2196-23 Revision
May 1, 2022 E2196-22 Revision
April 1, 2017 E2196-17 Revision
April 1, 2012 E2196-12 Revision
April 1, 2007 E2196-07 Revision
April 10, 2002 E2196-02 Revision