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ASTM E1200-24

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Standard Practice for Preserving Zooplankton Samples

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1.1 This practice describes the proper procedures for preserving zooplankton samples with either formaldehyde, ethanol, glutaraldehyde, Lugol’s iodine solution, or vinegar (acetic acid).

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


5.1 Calcium Carbonate (CaCO3) buffered formalin (3 % to 5 %) can be used as a permanent preservative for zooplankton. Lugol’s iodine solution can be used to preserve zooplankton for up to one year. Thirty percent ethanol, 30 % glutaraldehyde, or 25 % vinegar (can use 3 % acetic acid solution) can be used for more temporary storage and preservation of zooplankton samples. A 25 % vinegar solution is preferred to preserve soft-bodied planktonic coelenterates.

SDO ASTM: ASTM International
Document Number E1200
Publication Date April 1, 2024
Language en - English
Page Count 2
Revision Level 24
Supercedes
Committee D19.24
Publish Date Document Id Type View
April 1, 2024 E1200-24 Revision
April 1, 2019 E1200-19 Revision
Dec. 1, 2012 E1200-87R12 Reaffirmation
April 1, 2004 E1200-87R04 Reaffirmation
Oct. 10, 1998 E1200-87R98 Reaffirmation