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ASTM D8238-24

Current Revision

Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins

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1.1 This test method covers an immunological method to determine the amount of protein in Guayule Natural Rubber (GNR) and its products using rabbit antisera specific for Guayule Natural Rubber latex proteins. This immunoassay procedure quantitatively measures the level of GNR proteins in solution using an inhibition format. The samples may include but are not restricted to, gloves or other GNR product extracts, and liquid GNR samples which have been collected in order to measure the protein levels.

1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3 This test method can be used to determine the presence of GNR protein contamination on test items.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


5.1 NRL derived from Hevea contains over 200 proteins a number of which are allergens capable of eliciting Type 1 anaphylactic reactions. Guayule Natural Rubber derived from the Parthenium argentatum plant contains many fewer proteins and at much lower levels than Hevea NRL. While there have been no reports of allergic reactions to GNR proteins at present, they have been shown to induce antibody production in exposed individuals.4 An immunological assay that tests for the presence of GNR has the advantage of providing the specificity and sensitivity that other ASTM standards such as Test Methods D5712 and D6499 do not offer.

5.2 This test method describes an immunological method for the quantitation of Guayule natural rubber proteins using rabbit anti-GNR serum. Rabbits immunized with GNR proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all of the proteins in GNR. This test method may be used to determine if a test item contains material derived from a natural source.

SDO ASTM: ASTM International
Document Number D8238
Publication Date May 1, 2024
Language en - English
Page Count 6
Revision Level 24
Supercedes
Committee D11.40
Publish Date Document Id Type View
May 1, 2024 D8238-24 Revision
Dec. 1, 2018 D8238-18 Revision
May 1, 2023 D8238-18R23 Reaffirmation