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ASTM D7644-10e2

Historical Revision

Standard Test Method for Determination of Bromadiolone, Brodifacoum, Diphacinone and Warfarin in Water by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

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1.1 This procedure covers the determination of bromadiolone, brodifacoum, diphacinone and warfarin (referred to collectively as rodenticides in this test method) in water by direct injection using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS). These analytes are qualitatively and quantitatively determined by this method. This method adheres to multiple reaction monitoring (MRM) mass spectrometry.

1.3 UnitsThe values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

TABLE 1 Detection Verification Level and Reporting Range

AnalyteDVL
(ng/L)
Reporting Range
(ng/L)
Bromadiolone20125-2500
Brodifacoum20125-2500
Diphacinone20125-2500
Warfarin20125-2500


FIG. 1 Example Primary SRM Chromatograms Signal/Noise Ratios


FIG. 2 Example Confirmatory SRM Chromatograms Signal/Noise Ratios


This test method has been developed by US EPA Region 5 Chicago Regional Laboratory (CRL).

Bromadiolone, brodifacoum, diphacinone and warfarin are rodenticides for controlling mice, rats, and other rodents that pose a threat to public health, critical habitats, native plants and animals, crops, food and water supplies. These rodenticides also present human and environmental safety concerns. Warfarin and diphacinone are first-generation anticoagulants, while bromadiolone and brodifacoum are second-generation. The anticoagulants interfere with blood clotting, and death can result from excessive bleeding. The second-generation anticoagulants are especially hazardous for several reasons. They are highly toxic and persist a long time in body tissues. The second-generation anticoagulants are designed to be toxic in a single feeding, but time-to-death occurs in several days. This allows rodents to feed multiple times before death, leading to carcasses containing residues that may be many times the lethal dose.

This method has been investigated for use with reagent, surface, and drinking water for the selected rodenticides.

SDO ASTM: ASTM International
Document Number D7644
Publication Date June 1, 2010
Language en - English
Page Count 15
Revision Level 10e2
Supercedes
Committee D19.06
Publish Date Document Id Type View
April 15, 2023 D7644-23 Revision
Feb. 1, 2016 D7644-16 Revision
June 1, 2010 D7644-10E02 Revision
June 1, 2010 D7644-10E01 Revision
June 1, 2010 D7644-10 Revision