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ASTM D7304-06

Historical Revision

Standard Test Method for Determination of Denatonium Benzoate in Engine Coolant by HPLC

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1.1 This test method covers the chemical analysis of engine coolant for denatonium ion by high-performance liquid chromatography (HPLC).

1.2 This test method is applicable to both new and used coolants.

1.3 Coelution of other ions may cause interferences in the detection of the denatonium cation. In the case of unfamiliar formulations, identification verification should be performed by either or both fortification and dilution of the sample matrix with denatonium ion..

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


This test method provides for the qualitative and quantitative determination of denatonium ion in engine coolant in milligrams per liter to low percent range and requires approximately 100 mL per test, with results available in less than 30 min. Acceptable levels of denatonium vary with manufacturers blending specifications and applicable minimum or maximum industry and state specifications. Typically specification ranges from 30 to 50 ppm denatonium ion.

SDO ASTM: ASTM International
Document Number D7304
Publication Date Nov. 1, 2006
Language en - English
Page Count 5
Revision Level 06
Supercedes
Committee D15.04
Publish Date Document Id Type View
Sept. 1, 2022 D7304-22A Revision
March 1, 2022 D7304-22 Revision
May 15, 2016 D7304-16 Revision
March 15, 2014 D7304-14 Revision
Nov. 1, 2006 D7304-06 Revision