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ASTM D7304-22a

Current Revision

Standard Test Method for Determination of Denatonium Benzoate in Engine Coolant by HPLC

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1.1 This test method covers the chemical analysis of engine coolant for denatonium benzoate (DNB) by high-performance liquid chromatography (HPLC). DNB is added to potentially render engine coolant unpalatable to animals and humans. This analytical method was designed for the analysis of DNB and is not valid for any other bittering agents such as denatonium saccharide.

1.2 This test method is applicable to both new and used coolants.

1.3 Coelution of other ions may cause interferences in the detection of the denatonium cation. In the case of unfamiliar formulations, identification verification should be performed by either or both fortification and dilution of the sample matrix with denatonium ion.

1.4 The values stated in SI units are to be regarded as standard. The values given in parentheses are for information only.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


4.1 This test method provides for the qualitative and quantitative determination of denatonium benzoate in engine coolant in milligrams per litre to low percent range and requires approximately 1 mL per test, with results available in less than 10 min. Denatonium benzoate is a compound composed of a quaternary ammonium cation, denatonium and an inert anion, benzoate. In solution the denatonium benzoate exists in equilibrium between the denatonium benzoate compound, the denatonium cation and benzoate anion. By slightly adjusting the pH of the solution to be more acidic ( pH 4.6) the equilibrium will be shifted to the direction of forming more denatonium and benzoate ions in the solution.

SDO ASTM: ASTM International
Document Number D7304
Publication Date Sept. 1, 2022
Language en - English
Page Count 9
Revision Level 22a
Supercedes
Committee D15.04
Publish Date Document Id Type View
Sept. 1, 2022 D7304-22A Revision
March 1, 2022 D7304-22 Revision
May 15, 2016 D7304-16 Revision
March 15, 2014 D7304-14 Revision
Nov. 1, 2006 D7304-06 Revision