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ASTM D6503-99(2005)

Historical Reaffirmation

Standard Test Method for Enterococci in Water Using Enterolert

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1.1 This test method covers a simple procedure for the detection of enterococci in water and wastewater. It is based on IDEXX's patented Defined Substrate Technology (DST). This product, Enterolert, utilizes a nutrient indicator that fluoresces when metabolized. It can detect these bacteria at one colony forming unit (CFU)/100 mL within 24 h. The presence of this microorganism in water is an indication of fecal contamination and the possible presence of enteric pathogens.

1.2 This test method can be used successfully with drinking water, source water, recreational (fresh and marine) water, and bottled water. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


This test provides an easy and reliable method for the detection of enterococci in water within 24 h. For recreational water (fresh and marine) testing is performed to insure areas are safe for swimming. Enterolert also can be used for testing bottled water and drinking water.

SDO ASTM: ASTM International
Document Number D6503
Publication Date June 1, 2005
Language en - English
Page Count 7
Revision Level 99(2005)
Supercedes
Committee D19.24
Publish Date Document Id Type View
April 1, 2024 D6503-24 Revision
April 1, 2019 D6503-19 Revision
Aug. 1, 2014 D6503-14 Revision
Dec. 10, 1999 D6503-99 Revision
May 1, 2009 D6503-99R09 Reaffirmation
June 1, 2005 D6503-99R05 Reaffirmation