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ASTM D6499-00

Historical Revision

Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products

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1.1 This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


SDO ASTM: ASTM International
Document Number D6499
Publication Date Jan. 10, 2000
Language en - English
Page Count 6
Revision Level 00
Supercedes
Committee D11.40
Publish Date Document Id Type View
May 1, 2024 D6499-24 Revision
Aug. 1, 2018 D6499-18 Revision
July 1, 2016 D6499-16 Revision
May 1, 2012 D6499-12 Revision
Nov. 1, 2007 D6499-07 Revision
June 10, 2003 D6499-03 Revision
Jan. 10, 2000 D6499-00 Revision
May 1, 2023 D6499-18R23 Reaffirmation