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ASTM D5246-92(2004)

Current Reaffirmation

Standard Test Method for Isolation and Enumeration of <emph type="ital">Pseudomonas aeruginosa</emph> from Water

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1.1 This test method covers the isolation and enumeration of Pseudomonas aeruginosa (P. aeruginosa) from surface waters; recreational waters; ground water, water supplies; especially rural nonchlorinated sources; waste water; and saline waters. The detection limit of this test method is one microorganism per 100 mL.

1.2 This test method was used successfully with reagent water and it is the user's responsibility to ensure the validity of this test method for waters of untested matrices.

1.3 The values stated in SI units are to be regarded as the standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 10.


Pseudomonas aeruginosa is an opportunistic pathogen, and has been linked as the causative agent of numerous infections that may be transmitted through a contaminated water supply to a susceptible host. In addition to its direct pathogenicity, the association of P. aeruginosa with human fecal waste indicates that where elevated levels of P. aeruginosa are found, a serious health hazard may exist due to the presence of other pathogens.

The membrane filtration procedure described is a rapid and reliable test method of detecting P. aeruginosa in water.

SDO ASTM: ASTM International
Document Number D5246
Publication Date June 1, 2004
Language en - English
Page Count 4
Revision Level 92(2004)
Supercedes
Committee D19.24
Publish Date Document Id Type View
April 1, 2024 D5246-24 Revision
Dec. 1, 2019 D5246-19 Revision
June 1, 2015 D5246-15 Revision
May 1, 2013 D5246-13 Revision
June 1, 2004 D5246-92R04 Reaffirmation
Dec. 10, 1998 D5246-92R98 Reaffirmation