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ASTM D4455-85(1997)

Historical Reaffirmation

Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure (Withdrawn 2019)

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1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.

1.2 Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

1.3 The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.

1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5 Approximately 1014 cells/mL are required for detection by this test method.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


SDO ASTM: ASTM International
Document Number D4455
Publication Date Dec. 10, 1997
Language en - English
Page Count 3
Revision Level 85(1997)
Supercedes
Committee D19.24
Publish Date Document Id Type View
Jan. 1, 2014 D4455-85R14 Revision
May 1, 2009 D4455-85R09 Reaffirmation
Dec. 10, 1997 D4455-85R97 Reaffirmation
Jan. 25, 1985 D4455-85R02 Reaffirmation