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ASTM D4012-81(1997)

Historical Reaffirmation

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

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1.1 This test method covers the measurement of adenosine triphosphate (ATP) in microorganisms in concentrations normally found in laboratory cultures, water, wastewaters, and in plankton and periphyton samples from waters.

1.2 Knowledge of the concentration of ATP can be related to viable biomass or metabolic activity, or by utilizing an average concentration (or amount) of ATP per cell, an estimated count of microorganisms can be obtained in the case of unispecies cultures.

1.3 This test method offers a high degree of sensitivity, rapidity, accuracy, and reproducibility. However, extreme care must be taken at each step in the analysis to ensure meaningful and reliable results.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


SDO ASTM: ASTM International
Document Number D4012
Publication Date Dec. 10, 1997
Language en - English
Page Count 4
Revision Level 81(1997)
Supercedes
Committee D19.24
Publish Date Document Id Type View
Nov. 1, 2023 D4012-23A Revision
July 1, 2023 D4012-23 Revision
July 1, 2015 D4012-15 Revision
May 1, 2009 D4012-81R09 Reaffirmation
Dec. 10, 1997 D4012-81R97 Reaffirmation
May 29, 1981 D4012-81R02 Reaffirmation