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ASTM D3862-80(2001)

Historical Revision

Standard Test Method for Retention Characteristics of 0.2-µm Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality

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1.1 This test method covers a procedure to test membrane filters for their ability to retain bacteria whose diameter is equal to or slightly larger than the 0.2-µm pore size of the membrane filter.

1.2 The procedures described are for the use of user laboratories as differentiated from manufacturers' laboratories.

1.3 This standard does not purport to address the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


Microbiological water testing procedures using membrane filtration are based on the premise that all bacteria within a specific size range will be retained by the membrane filter used. If the membrane filter does not retain these bacteria, false negative results or lowered density estimates may occur that could have serious repercussions due to the presence of unrecognized potential health hazards in the water being tested, especially in drinking water.

5.1.1 This procedure as devised will enable the user to test each membrane filter lot number for its ability to retain all bacterial equal to, or larger than, the stated membrane pore size.

Since this membrane is often used to sterilize nonautoclavable liquids, it is essential that the retention characteristics of this membrane are stable.

SDO ASTM: ASTM International
Document Number D3862
Publication Date Jan. 1, 2001
Language en - English
Page Count 3
Revision Level 80(2001)
Supercedes
Committee D19.08
Publish Date Document Id Type View
Feb. 15, 2013 D3862-13 Revision
Jan. 1, 2001 D3862-80R01 Revision
Nov. 1, 2024 D3862-13R24 Reaffirmation
Nov. 1, 2019 D3862-13R19 Reaffirmation