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ASTM D3598-89(2009)

Historical Reaffirmation

Standard Test Method for Citrate in Synthetic Detergents

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1.1 This test method covers the enzymatic determination of citrate in both liquid and solid synthetic detergents. The test method is applicable to most detergents containing citrate at a minimum concentration of approximately 5 % (1-8).

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Material Safety Data Sheets are available for reagents and materials. Review them for hazards prior to usage.

This test method covers the enzymatic determination of citrate in both liquid and solid synthetic detergents. The test method is applicable to most detergents containing citrate at a minimum concentration of approximately 5 %. The apparatus to be used in the determination of citrate shall includes interval timer, micropipet, and spectrophotometer. An enzyme system that is based upon the selective cleavage of citrate by citrate lyase (citrate oxaloacetate-lyase; EC 4.1.3.6) shall be employed. One of the products, oxaloacetate, shall be reduced to malate by malic dehydrogenase (L-malate: NAD oxidoreductase; EC 1.1.1.37) with the simultaneous oxidation of reduced (beta)-nicotinamide adenine dinucleotide to (beta)-nicotinamide adenine dinucleotide, oxidized form. The course of the reaction shall be measured spectrophotometrically. The decrease in absorbance at 340 nm caused by the formation of (beta)-nicotinamide adenine dinucleotide, oxidized form, is directly proportional to the concentration of citrate.


SDO ASTM: ASTM International
Document Number D3598
Publication Date Oct. 1, 2009
Language en - English
Page Count 3
Revision Level 89(2009)
Supercedes
Committee D12.12
Publish Date Document Id Type View
Nov. 1, 2024 D3598-89R24 Reaffirmation
July 1, 2016 D3598-89R16 Reaffirmation
Oct. 1, 2009 D3598-89R09 Reaffirmation
Jan. 1, 1995 D3598-89R95E01 Reaffirmation
May 26, 1989 D3598-89R03 Reaffirmation